Not known Details About thr77
Not known Details About thr77
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is noticed in lots of tumors, which leads to chromosome instability, and so might lead to tumorigenesis. This protein was found to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant part in endorsing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already explained. Two extra splice variants had been documented but specific nucleotide sequence information and facts isn't nevertheless offered.
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Blocking peptides are peptides that bind precisely on the goal antibody and block antibody binding. These peptide generally incorporates the epitope identified through the antibody.
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This guidebook outlines additional possible optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This manual outlines further doable optimization of cellular and immunoassay parameters to ensure the absolute best final results are...
Antibodies certain to the blocking peptide no more bind for the epitope to the target protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is certain.
Target Facts Cyclin E belongs towards the highly conserved cyclin spouse and children, whose associates are characterized by a dramatic periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation patterns which contribute on the temporal coordination of each and every mitotic event. Cyclin E sorts a complex with and capabilities as a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates within the G1-S phase boundary and is also degraded as cells progress by means of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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